About BioPRYN and Pregnancy Testing
Q. What is BioPRYN?
A. BioPRYN is a blood pregnancy test for cattle, bison, sheep and goats, and has specific appeal for the commercial U.S. dairy industry, because it delivers fast, accurate, safe and economical pregnancy diagnostic results.
Q. What does the test name stand for?
A. The test's name is a partial acronym for "Pregnant Ruminant Yes/No". This specific test using ELISA technology, which produces fast results, has been develop for use exclusively in cattle.
Q. How does BioPRYN detect pregnancies?
A. BioPRYN evaluates the blood (more specifically, the serum or plasma) of ruminants for a protein called Pregnancy Specific Protein B (PSPB). PSPB is produced by the placenta, and therefore pregnant animals will have the protein in their blood. This makes the test more accurate than earlier attempts at pregnancy diagnosis that evaluated blood or milk for progesterone or other hormones that can occur in normally cycling animals. The test uses enzyme-linked immunosorbent assay (ELISA) technology for processing, which contributes to its low cost and fast turn-around.
Q. How early in a pregnancy can animals be tested with BioPRYN?
A. Adult cattle can be tested for pregnancy 28 or more days following insemination, virgin heifers can be tested 25 days or more after insemination.. Animals that are detected open can then be immediately returned to time saving re-synchronization protocols using products such as Estroplan and Gonabreed.
Q. What is the accuracy of BioPRYN?
A. The test is >99 percent accurate in detecting open cows, with only <1 percent showing false-open (false-negative). Correct open detection is very important when giving Estroplan or other synchronizing drugs.
Q. What are the advantages of using blood testing versus other methods of pregnancy diagnosis?
Q. How long does it take to receive the test results?
- Producers incur less cost using BioPRYN compared to rectal palpation by a veterinarian, and much less cost than ultrasound.
- Blood testing allows producers to diagnose pregnancy at least 7 days sooner than rectal palpation, with no risk of damaging the fetus.
- Cows diagnosed open can then be re-bred sooner, resulting in tighter calving intervals, more calves born per year, and higher lifetime milk production (because cows achieve peak milk more often).
- The test is more convenient to dairies because on-farm personnel can draw samples without locking up cows or waiting for the veterinarian to show upl and ship blood samples,
- Eliminating rectal palpation also frees up the veterinarian to concentrate on higher-level issues with the dairy's management team.
A. The test requires 48 hours from laboratory set-up to reporting. Reports are faxed or e-mailed back to the dairy.
Q. How should blood samples be prepared and shipped?
A. Dairy personnel need to draw blood samples from the tail vein, which is an easy-to-learn procedure. How to Tail Bleed Blood samples of at least 2 mL per animal should be collected in individual vacuum tubes and labeled with each animal's identification number. It is important to draw samples using individual, disposable needles, to avoid cross-contamination between animals. Tubes containing blood samples should not be opened and should be packed in a well-padded box to avoid breakage. They do NOT need to be packaged in ice. Samples may stay in transit for several days without compromising the results of the test. Fastest results, however, are achieved when samples are shipped via Federal Express, UPS overnight, DHL, Airborne Express, U.S. postal service overnight, or other overnight carrier method.
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